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Post by himike on Jul 15, 2014 3:13:11 GMT -5
Table S1. Yeast cells were developed at 30 C in either rich YPD or in a synthetic dextrose dium supplented with needed vitamins and minerals as advisable ray ban sunglasses outlet , For strong plates, two% agar was extra to possibly YPD or SD dium prior to autoclaving. Yeast cells were trans ford employing a LiAc technique as explained, The supply and preparation of ubc13..HIS3 cas settes was as beforehand explained, Cloning rice UBC13 cDNA and plasmid building To clone the entire duration open studying body of OsUBC13, total RNA was isolated from rice seedlings by employing TRIzol reagents for RT PCR with the RevertAid Very first Strand cDNA Synthesis Kit adhering to the panies protocols. OsUbc13 specific prirs are as stick to. 5 aatggaattc ATGGCCAACAGCAACCTC three and five atgcgtcgT TATGCACCGCTGGC 3. The ahead prir contains an EcoRI restriction inter site and the reverse prir consists of a SalI site, as underlined. The PCR solution of OsUBC13 ORF was cloned into yeast two hybrid vecto
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